Determination of circulating Mycobacterium tuberculosis strains and transmission patterns among TB patients in Iran, using 15 loci MIRU-VNTR
نویسندگان
چکیده
منابع مشابه
Synchronous Comparison of Mycobacterium tuberculosis Epidemiology Strains by “MIRU-VNTR” and “MIRU-VNTR and Spoligotyping” Technique
Molecular epidemiology analyses are frequently used in determining epidemiology of tuberculosis. Recently, Mycobacterial Interspersed Repetitive Unit Variable Number Tandem Repeat (MIRU-VNTR) and Spoligotyping has become an important method, as it allows high-through put, discriminatory and reproducible analysis of clinical isolate. The purpose of this study is to compare techniques of "MIRU-VN...
متن کاملDetermining the Risk of Intra-Community Transmission of Tuberculosis in the Northwest of Iran Through 15 Loci Miru-Vntr Typing
This study was carried out in order to investigating the effect of travelling on the transmission of tuberculosis from high- to low-burden TB countries. Mycobacteria samples isolated from patients of distinct and relatively co-related countries (Azerbaijan Republic and Tabriz [located in the northwest of Iran]) were analyzed through 15 loci MIRU-VNTR typing method. PCR was done using special pr...
متن کاملتعیین ساختار ژنتیکی مایکوباکتریوم توبرکلوزیس به روش MIRU- VNTR 15 loci
Background and purpose: Tuberculosis is among the leading causes of death from infectious diseases in the world. According to World Health Organization (WHO), the estimated rate of TB in Iran was 21 per 100,000 populations in 2015. The present study was designed to evaluate the genetic diversity and transmission dynamics of Mycobacterium tuberculosis in Tehran, Iran. Materials and methods: A...
متن کاملDetection of multiple strains of Mycobacterium tuberculosis using MIRU-VNTR in patients with pulmonary tuberculosis in Kampala, Uganda
BACKGROUND Many studies using DNA fingerprinting to differentiate Mycobacterium tuberculosis (MTB) strains reveal single strains in cultures, suggesting that most disease is caused by infection with a single strain. However, recent studies using molecular epidemiological tools that amplify multiple targets have demonstrated simultaneous infection with multiple strains of MTB. We aimed to determ...
متن کاملMIRU-VNTR Genotyping of Mycobacterium tuberculosis Strains Using QIAxcel Technology: A Multicentre Evaluation Study
BACKGROUND Molecular genotyping of M.tuberculosis is an important laboratory tool in the context of emerging drug resistant TB. The standard 24-loci MIRU-VNTR typing includes PCR amplification followed by the detection and sizing of PCR fragments using capillary electrophoresis on automated sequencers or using agarose gels. The QIAxcel Advanced system might offer a cost-effective medium-through...
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ژورنال
عنوان ژورنال: International Journal of Mycobacteriology
سال: 2015
ISSN: 2212-5531
DOI: 10.1016/j.ijmyco.2014.11.051